Identification of Monoclonal Anti-HMW-MAA Antibody Linear Peptide Epitope by Proteomic Database Mining

Characterisation of anti-IgG monoclonal antibody A57H by epitope mapping.

Monoclonal antibodies have proved powerful tools in serological techniques and as probes for elucidating the antigenic determinants of macromolecules and cells. However an inherent problem of these reagents is the phenomenon of assay restriction, where a given antibody may perform well in some assays, whilst being poor or ineffective in others [I] . Thus stringent evaluation of a monoclonal ant...

Identification of peptides mimicking the natural epitope recognized by the CB-EGF1 anti-EGF monoclonal antibody

As part of an integral structure and function study, we characterized the natural epitope on the EGF recognized by the CB-EGF1 monoclonal antibody, by using peptides displayed on the coat protein of filamentous bacteriophages. Data analyzed demonstrated that the CB-EGF1 monoclonal antibody recognizes a conformational epitope, comprising aminoacids S9, H10, Y13, K28, E40 and R41. The identified ...

Precise epitope determination of the anti-vimentin monoclonal antibody V9

Vimentin is a type III intermediate filament protein that is typically expressed in mesenchymal cells. Overexpression of vimentin is frequently observed in several types of cancer and is often associated with epithelial‑to‑mesenchymal transition. It was recently reported that the serum vimentin level is significantly elevated in colon and liver tumors. Therefore, a more sensitive vimentin detec...

Development of a Novel Inhibitory Chimeric Anti-HER2 Monoclonal Antibody

Antibodies in Patients with Melanoma Lymphocytes Elicited by Anti-Idiotypic Monoclonal Weight-Melanoma-Associated Antigen-Specific Cytotoxic T Fine Specificity of High Molecular

HLA-A2-restricted CTLs, which lysed high molecular weight (HMW)melanoma-associated antigen (MAA) melanoma cells, were induced in patients with melanoma immunized with MELIMMUNE, a combination of the murine anti-idiotypic (anti-id) monoclonal antibodies (mAb) MEL-2 and MF11–30 (MW Pride et al., Clin Cancer Res 1998;4:2363.). In the present study we investigated whether CTL epitopes are present i...